* The data is last updated on 10th February, 2023 from GISAID, based on 2379 variants from (14.9 million sequences) and 34 primer sets.
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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 2379 SARS-CoV-2 variants.

Lineage
(WHO label)
  
Target Gene
  
Primer Name
  
Primer Sequence
  
Primer Length
  
Primer GC Content (%)
  
Start Position
  
End Position
  
Primer Direction
  
ΔG (cal)
  
Primer first used
  
BA.1.13.1ORF1abWon-ORF1abTGCATTAACATTGGCCGTGA20451555815539R-18602South Korea
BA.1.13.1ECharité-EATATTGCAGCAGTACGCACACA2245.452638126360R-20682.9Germany
B.1.1.7 (Alpha)ECharité-EATATTGCAGCAGTACGCACACA2245.452638126360R-20682.9Germany
BA.1.13.1SYoung-SATCCAGCCTCTTATTATGTTAGAC2437.52187621853R-18955.1Singapore
B.1.1.7 (Alpha)EHuang-EGCAGCAGTACGCACACAATC20552637626357R-20966.7China
B.1.1.7 (Alpha)SNIID WH-1 Seq F24383R24865CAAAGACACCTTCACGAGG1952.632484824830R-18239.2Japan
B.1.1.7 (Alpha)ENiu-ECACGTTAACAATATTGCAGC20402639126372R-18150China
B.1.1.7 (Alpha)SWuhanCoV-spk1TGTGGTTCATAAAAATTCCTTTGTG25322490024876R-20606.6Japan
B.1.1.7 (Alpha)NCN-CDC-NCAGACATTTTGCTCTCAAGCTG2245.452897928958R-16971.2China
B.1.1.7 (Alpha)NSC2CCTTGTGTGGTCTGCATGAGTTTAG25482955429530R-23231.2US
B.1.1.7 (Alpha)NNIH-TH_NCCCCACTGCGTTCTCCATT1957.892837628358R-19688.1Thailand
BA.1.13.1ORF1abPasteur-ORF1ab-1CTCCCTTTGTTGTGTTGT1844.441279712780R-16534.9France
B.1.1.7 (Alpha)NUS-CDC-N-1TCTGGTTACTGCCAGTTGAATCTG2445.832835828335R-22032.7US
B.1.1.7 (Alpha)EWon-ECACACAATCGATGCGCAGTA20502636526346R-16823.1South Korea
B.1.1.7 (Alpha)NUS-CDC-N-2GCGCGACATTCCGAAGAA1855.562923029213R-19101.3US
BA.1.13.1ORF1abYoung-ORF1abCACTTAATGTAAGGCTTTGTTAAG2433.331424314220R-19292.3Singapore
B.1.1.7 (Alpha)NUS-CDC-N-3TGTAGCACGATTGCAGCATTG2147.622875228732R-21070.3US
BA.1.13.1ORF1abYip-ORF1abTTGTTATAGCGGCCTTCTGT204519701951R-2957.93China
B.1.1.7 (Alpha)NYoung-NAGCACCATAGGGAAGTCC1855.562864828631R-17571.6Singapore
B.1.1.7 (Alpha)NChan-NTTGGATCTTTGTCATCCAATTTG2334.782930629284R-14526.9China
B.1.1.7 (Alpha)NCorman-NGAGGAACGAGAAGAGGCTTG20552883328814R-19953.7Germany
BA.1.13.1ORF1abHKU-ORF1bAACRCGCTTAACAAAGCACTC2142.861890918889R-15488.5Hong Kong
B.1.1.7 (Alpha)NWon-NGTTGCGACTACGTGATGAGG20552884928830R-20514.2South Korea
BA.1.13.1ORF1abChan-ORF1abGTGTGATGTTGAWATGACATGGTC2441.671635316330R-10608.2China
B.1.1.7 (Alpha)NNIID-JP-NTGGCAGCTGTGTAGGTCAAC20552928229263R-18084Japan
B.1.1.7 (Alpha)NHKU-NCGAAGGTGTGACTTCCATG1952.632925429236R-18498.1Hong Kong
B.1.1.7 (Alpha)ORF1abWon-ORF1abTGCATTAACATTGGCCGTGA20451555815539R-18602South Korea
B.1.1.7 (Alpha)ORF1abYip-ORF1abTTGTTATAGCGGCCTTCTGT204519701951R-18501.6China
B.1.1.7 (Alpha)ORF1abNIID WH-1 F509R854CAGAAGTTGTTATCGACATAGC2240.91837816R-18413.1Japan
B.1.1.7 (Alpha)ORF1abPasteur-ORF1ab-1CTCCCTTTGTTGTGTTGT1844.441279712780R-13463.1France
BA.1.13.1SChan-SCAAGCTATAACGCAGCCTGTA2147.622286922849R-19364.2China
B.1.1.7 (Alpha)ORF1abPasteur-ORF1ab-2CTGGTCAAGGTTAATATAGG20401418614167R-15775France
BA.1.13.1ORF1abPasteur-ORF1ab-2CTGGTCAAGGTTAATATAGG20401418614167R-15775France
B.1.1.7 (Alpha)ORF1abCN-CDC-ORF1abACGATTGTGCATCAGCTGA1947.371346013442R-17867.8China
B.1.1.7 (Alpha)SNIID WH-1 F24381R24873ATTTGAAACAAAGACACCTTCAC2334.782485624834R-19212.9Japan
B.1.1.7 (Alpha)ORF1abYoung-ORF1abCACTTAATGTAAGGCTTTGTTAAG2433.331424314220R-19292.3Singapore
BA.1.13.1EHuang-EGCAGCAGTACGCACACAATC20552637626357R-20966.7China
B.1.1.7 (Alpha)ORF1abCharité-ORF1bCARATGTTAAASACACTATTAGCATA2623.081553015505R-6346.39Germany
BA.1.13.1EWon-ECACACAATCGATGCGCAGTA20502636526346R-16823.1South Korea
N/ASNIID WH-1 Seq F24383R24865CAAAGACACCTTCACGAGG1952.632484824830R-18239.2Japan
B.1.1.7 (Alpha)ORF1abNIID WH-1 Seq F519R840GACATAGCGAGTGTATGCC1952.63823805R-17726.8Japan
B.1.1.7 (Alpha)ORF1abChan-ORF1abGTGTGATGTTGAWATGACATGGTC2441.671635316330R-10608.2China
BA.1.13.1ORF1abCharité-ORF1bCARATGTTAAASACACTATTAGCATA2623.081553015505R-6346.39Germany
B.1.1.7 (Alpha)ORF1abHKU-ORF1bAACRCGCTTAACAAAGCACTC2142.861890918889R-15488.5Hong Kong
BA.1.13.1ORF1abCN-CDC-ORF1abACGATTGTGCATCAGCTGA1947.371346013442R-17867.8China
B.1.1.7 (Alpha)SYoung-SATCCAGCCTCTTATTATGTTAGAC2437.52187621853R-18955.1Singapore
B.1.1.7 (Alpha)ORF1abNIID WH-1 F501R913CTTTACCAGCACGTGCTAGAAGG2352.17896874R-22677.2Japan
B.1.1.7 (Alpha)SChan-SCAAGCTATAACGCAGCCTGTA2147.622286922849R-19364.2China
BA.1.13.1SWon-SCACCTGTGCCTGTTAAACCA20502321323194R-16003.5South Korea
B.1.1.7 (Alpha)SWon-SCACCTGTGCCTGTTAAACCA20502321323194R-19392.1South Korea
Lineage
(WHO label)
  
Target Gene
  
Primer Name
  
Primer Sequence
  
Primer Length
  
Primer GC Content (%)
  
Start Position
  
End Position
  
Primer Direction
  
ΔG (cal)
  
Primer first used