* The data is last updated on 10th February, 2023 from GISAID, based on 2379 variants from (14.9 million sequences) and 34 primer sets.
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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 2379 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.7 (Alpha) | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| BA.2 (Omicron) | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| AY.100 (Delta) | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -19979.3 | US |
| BA.2 (Omicron) | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.177.7 | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| BA.1.13.1 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| B.1.1.7 (Alpha) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -23231.2 | US |
| B.1.1.7 (Alpha) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| AY.100 (Delta) | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| AY.100 (Delta) | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| AY.100 (Delta) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| B.1.1.7 (Alpha) | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.1.7 (Alpha) | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| B.1.525 (Eta) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| B.1.1.7 (Alpha) | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| B.1.1.7 (Alpha) | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -19979.3 | US |
| B.1.1.7 (Alpha) | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| B.1.177.7 | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| BA.1.15 | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.177.7 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| BA.1.15 | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| B.1.525 (Eta) | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.525 (Eta) | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| B.1.525 (Eta) | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| BA.1.13.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| BA.1.13.1 | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.525 (Eta) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -19265.8 | US |
| BA.2 (Omicron) | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -19979.3 | US |
| BA.2 (Omicron) | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| BA.2 (Omicron) | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| BA.2 (Omicron) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| AY.100 (Delta) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -23231.2 | US |
| BA.1.13.1 | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| BA.1.13.1 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| B.1.177.7 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -20137 | US |
| B.1.177.7 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -23231.2 | US |
| B.1.177.7 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| BA.2 (Omicron) | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| B.1.525 (Eta) | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -21070.3 | US |
| BA.2 (Omicron) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -23231.2 | US |
| BA.1.13.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -23231.2 | US |
| BA.1.13.1 | N | US-CDC-N-2 | GCGCGACATTCCGAAGAA | 18 | 55.56 | 29230 | 29213 | R | -19101.3 | US |
| B.1.177.7 | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| B.1.177.7 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -19979.3 | US |
| AY.100 (Delta) | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| BA.1.13.1 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -19979.3 | US |
| AY.100 (Delta) | N | US-CDC-N-1 | GACCCCAAAATCAGCGAAAT | 20 | 45 | 28287 | 28306 | F | -18082.8 | US |
| AY.100 (Delta) | N | US-CDC-N-1 | TCTGGTTACTGCCAGTTGAATCTG | 24 | 45.83 | 28358 | 28335 | R | -22032.7 | US |
| B.1.525 (Eta) | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -18541.7 | US |
| B.1.525 (Eta) | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -15759.5 | US |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |