Generate Primer Mutants (through single nucleotide deletion) and Predict their Binding Energy

Predict Primer mutants binding affinities on the template sequence binding region
Mutants module of AssayM allow users to generate Primer mutants (single substitution mutation at a time) and predict their binding affinities on the user-specified genomic sequence (Primer binding region).

Paste Primer Sequence (Max. length: 36 bp; to Generate Mutants):
   

Paste Template Sequence (Max. length: 150 bp; Primer binding region only):