Scan Genome Sequence (GS) with a Primer Sequence
Predict Primer's binding affinities on selected genome sequence (GS) regions
Genome Scan module of AssayM
allow users to predict the Primer's binding affinities on selected GS regions.
Paste Primer Sequence (Max. length: 36 bp):
User may submit
one genome sequence (GS)
using any of the two options provided. Please choose only one option at a time.
Option 1. Paste one genome sequence (in FASTA format only; only one sequence at a time)
[Click to see example sequence]
Option 2. Upload File (with one genome in FASTA format only; file maximum size 5 MB)
[Click to see example sequence]
Template Sequence Position (to be scanned by Primer):
Start Position:
End Position:
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Note:
Highlighted ΔG > 0 implies potential loss of sensitivity of the PCR primer for that specific variant
Apply Filters on the Results given below:
Minimum GC %:
Maximum GC %:
Minimum Delta G:
Maximum Delta G:
Download results
