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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| HN.7 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| HN.7 | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| HN.7 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| HN.7 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| GE.1.6 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| GE.1.6 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| GE.1.6 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| GE.1.6 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| GE.1.6 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| GE.1.6 | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| GE.1.6 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| GE.1.6 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| EG.5.1.9 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| EG.5.1.9 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| EG.5.1.9 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| EG.5.1.9 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| EG.5.1.9 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| EG.5.1.9 | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| EG.5.1.9 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| EG.5.1.9 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| XCH | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XCH | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| XCH | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XCH | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| XCH | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XCH | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| XCH | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| XCH | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| JF.3 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| JF.3 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| JF.3 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| JF.3 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| JF.3 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| JF.3 | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| JF.3 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| JF.3 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| DV.7.1.4 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| DV.7.1.4 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -15090.2 | South Korea |
| DV.7.1.4 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| DV.7.1.4 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -16141.5 | Germany |
| DV.7.1.4 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| DV.7.1.4 | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -12594.2 | China |
| DV.7.1.4 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| DV.7.1.4 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -3779.86 | China |
| XCL | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XCL | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| XCL | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XCL | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| XCL | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XCL | E | Huang-E | GCAGCAGTACGCACACAATC | 20 | 55 | 26376 | 26357 | R | -23540.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |