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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
AY.80 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
AY.80 (Delta)	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
AY.43.8 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.43.8 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-463.749	South Korea
AY.43.8 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.43.8 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-3196.01	Germany
AY.43.8 (Delta)	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-1339.23	China
AY.43.8 (Delta)	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-3384	China
AY.43.8 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-1427.97	China
AY.43.8 (Delta)	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-3779.86	China
AY.128 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.128 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.128 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.128 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.128 (Delta)	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-25022.8	China
AY.128 (Delta)	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-23540.5	China
AY.128 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
AY.128 (Delta)	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
A.21	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
A.21	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
A.21	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
A.21	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
A.21	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-25022.8	China
A.21	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-23540.5	China
A.21	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
A.21	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
AY.4.9 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.4.9 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
AY.4.9 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.4.9 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-3196.01	Germany
AY.4.9 (Delta)	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-25022.8	China
AY.4.9 (Delta)	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-3384	China
AY.4.9 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
AY.4.9 (Delta)	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-3779.86	China
B.1.398	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.398	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
B.1.398	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
B.1.398	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
B.1.398	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-25022.8	China
B.1.398	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-23540.5	China
B.1.398	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
B.1.398	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
AY.94 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.94 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.94 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.94 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.94 (Delta)	E	Huang-E	ACTTCTTTTTCTTGCTTTCGTGGT	24	37.5	26295	26318	F	-25022.8	China
AY.94 (Delta)	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-23540.5	China
AY.94 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-19913.4	China
AY.94 (Delta)	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used