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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
BA.5.2.32	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BA.5.2.32	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
BA.5.2.32	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
BA.5.2.32	ORF1ab	Yip-ORF1ab	TTGTTATAGCGGCCTTCTGT	20	45	1970	1951	R	-21075.5	China
BA.5.2.32	ORF1ab	Pasteur-ORF1ab-1	ATGAGCTTAGTCCTGTTG	18	44.44	12690	12707	F	-17837.6	France
BA.5.2.32	ORF1ab	Pasteur-ORF1ab-1	CTCCCTTTGTTGTGTTGT	18	44.44	12797	12780	R	-18837.8	France
BA.5.2.32	ORF1ab	Pasteur-ORF1ab-2	GGTAACTGGTATGATTTCG	19	42.11	14080	14098	F	-18581.4	France
BA.5.2.32	ORF1ab	Pasteur-ORF1ab-2	CTGGTCAAGGTTAATATAGG	20	40	14186	14167	R	-18348.8	France
BA.5.2.32	ORF1ab	CN-CDC-ORF1ab	CCCTGTGGGTTTTACACTTAA	21	42.86	13342	13362	F	-20516.2	China
BA.5.2.32	ORF1ab	CN-CDC-ORF1ab	ACGATTGTGCATCAGCTGA	19	47.37	13460	13442	R	-20306.2	China
BA.5.2.32	ORF1ab	Young-ORF1ab	TCATTGTTAATGCCTATATTAACC	24	29.17	14155	14178	F	-20695.4	Singapore
BA.5.2.32	ORF1ab	Young-ORF1ab	CACTTAATGTAAGGCTTTGTTAAG	24	33.33	14243	14220	R	-22408	Singapore
BA.5.2.32	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.5.2.32	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-9191.15	Germany
BA.5.2.32	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.5.2.32	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.5.2.32	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-11502.5	China
BA.5.2.32	ORF1ab	Chan-ORF1ab	GTGTGATGTTGAWATGACATGGTC	24	41.67	16353	16330	R	-13317.5	China
BA.5.2.32	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.2.32	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.2.32	S	Young-S	TATACATGTCTCTGGGACCA	20	45	21763	21782	F	-13305.5	Singapore
BA.5.2.32	S	Young-S	ATCCAGCCTCTTATTATGTTAGAC	24	37.5	21876	21853	R	-22070.7	Singapore
BA.5.2.32	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-27219.1	China
BA.5.2.32	S	Chan-S	CAAGCTATAACGCAGCCTGTA	21	47.62	22869	22849	R	-22073.5	China
BA.5.2.33	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
BA.5.2.33	ORF1ab	Yip-ORF1ab	TTGTTATAGCGGCCTTCTGT	20	45	1970	1951	R	-21075.5	China
BA.5.2.33	ORF1ab	Pasteur-ORF1ab-1	ATGAGCTTAGTCCTGTTG	18	44.44	12690	12707	F	-17837.6	France
BA.5.2.33	ORF1ab	Pasteur-ORF1ab-1	CTCCCTTTGTTGTGTTGT	18	44.44	12797	12780	R	-18837.8	France
BA.5.2.33	ORF1ab	Pasteur-ORF1ab-2	GGTAACTGGTATGATTTCG	19	42.11	14080	14098	F	-18581.4	France
BA.5.2.33	ORF1ab	Pasteur-ORF1ab-2	CTGGTCAAGGTTAATATAGG	20	40	14186	14167	R	-18348.8	France
BA.5.2.33	ORF1ab	CN-CDC-ORF1ab	CCCTGTGGGTTTTACACTTAA	21	42.86	13342	13362	F	-20516.2	China
BA.5.2.33	ORF1ab	CN-CDC-ORF1ab	ACGATTGTGCATCAGCTGA	19	47.37	13460	13442	R	-20306.2	China
BA.5.2.33	ORF1ab	Young-ORF1ab	TCATTGTTAATGCCTATATTAACC	24	29.17	14155	14178	F	-20695.4	Singapore
BA.5.2.33	ORF1ab	Young-ORF1ab	CACTTAATGTAAGGCTTTGTTAAG	24	33.33	14243	14220	R	-22408	Singapore
BA.5.2.33	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.5.2.33	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-9191.15	Germany
BA.5.2.33	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.5.2.33	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.5.2.33	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-11502.5	China
BA.5.2.33	ORF1ab	Chan-ORF1ab	GTGTGATGTTGAWATGACATGGTC	24	41.67	16353	16330	R	-13317.5	China
BA.5.2.33	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.2.33	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.2.33	S	Young-S	TATACATGTCTCTGGGACCA	20	45	21763	21782	F	-3872.72	Singapore
BA.5.2.33	S	Young-S	ATCCAGCCTCTTATTATGTTAGAC	24	37.5	21876	21853	R	-22070.7	Singapore
BA.5.2.33	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-27219.1	China
BA.5.2.33	S	Chan-S	CAAGCTATAACGCAGCCTGTA	21	47.62	22869	22849	R	-22073.5	China
BA.5.2.33	S	Won-S	CTACATGCACCAGCAACTGT	20	50	23114	23133	F	-22142.8	South Korea
BA.5.2.33	S	Won-S	CACCTGTGCCTGTTAAACCA	20	50	23213	23194	R	-21965.9	South Korea
BA.5.2.33	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.5.2.33	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used