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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BQ.1.23 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BF.11 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.12 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BF.7.15 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.3.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| DN.2 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.1.10 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.1.28 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| CJ.1.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XBF.6 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| BQ.1.1.15 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.1.31 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| GA.6 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XBB.1.5.12 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XBB.2.3.9 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| FD.4 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| CM.7 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.25.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.13.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| XBC.1.4 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| GC.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XBB.1.5.40 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| BQ.1.1.5 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| XBB.1.32 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| BQ.1.1.65 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| XCV | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| BA.2.31.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.2.50 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.2.78 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.2.43 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| HR.1.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HK.7 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HS.1.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HH.5 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HU.1.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HV.1.5 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| BA.5.2.54 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| DR.2 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BF.7.27 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| B.1.1.26 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| CM.8 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.5.1.27 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BF.16 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.5.5.3 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BA.5.2.59 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BF.15 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| BQ.1.15 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -22616.4 | Germany |
| B.1.160 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| JV.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XCY | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |