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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.75.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BQ.1.1.66 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BN.1.3.12 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.5.2.6 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| CH.1.1.13 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20849 | China |
| BA.2.9 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BM.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.5.1.5 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BQ.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| CH.1.1.15 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BQ.1.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| BQ.1.1.19 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BN.1.3.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.4.6.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.5.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.5.2.20 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BQ.1.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| DV.3 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.4.1.8 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BN.1.3 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| BA.5.1.22 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| BA.2.75 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| BA.5 (Omicron) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.5.5 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| CH.1.1.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GK.1.11.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| FL.32.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GA.8.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| HK.34 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| HC.3 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.93 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| FL.31.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GW.5.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| FL.38 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| EG.2.4 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.418 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.389 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.177.53 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.363 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| HV.1.7 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.405 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.282 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.228 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.174 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.88 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.106 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.346 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.93 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.512 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.349 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |