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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BG.4 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BF.9 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.4.1.9 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.576 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.325 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.22 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.521 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.378 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.206 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.1.403 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.636 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.126 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BQ.1.1.47 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XDQ.3 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| JD.1.1.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBC.1.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| FY.9 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBB.1.31.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| GK.1.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBB.1.5.27 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBB.9 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| CK.1.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| CM.8.1.3 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| CA.3.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.5.2.61 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBB.2.5 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| ED.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BU.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XBB.1.6 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BN.1.3.11 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| KB.4 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| HF.1.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BG.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| JN.1.42.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BQ.1.1.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.1.517 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.427 (Epsilon) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.429 (Epsilon) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| B.1.166 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.4.2 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| A.1 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| AY.16.1 (Delta) | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.2.9.7 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.2.72 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.2.7 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.2.20 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| BA.2.73 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XDZ | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| XEB | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -4388.79 | US |
| B.1.263 | N | SC2 | CCTTGTGTGGTCTGCATGAGTTTAG | 25 | 48 | 29554 | 29530 | R | -26482.3 | US |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |