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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.36 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.518 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.551 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| C.17 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| JC.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.564 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.461 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.486 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.603 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.70 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.606 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.601 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.186 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.543 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.139 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| C.31 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.221 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.362 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.265 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.585 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.351 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.36.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.36.8 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.466 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| L.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.339 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.229 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.415 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.370 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.421 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.428.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.612 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.305 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FY.1.2.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| JN.1.50 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| KP.2.14 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| unclassifiable | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| KS.1.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.1.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FY.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| HH.2.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XCE | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| HN.4.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| GU.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BE.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BR.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CH.1.1.28 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CH.1.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |