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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBB.1.19 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| KF.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CS.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.2.83 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BJ.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XDG | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GA.4 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BQ.1.26.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BQ.1.10.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XAY.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CY.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.3.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBT | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BQ.1.1.12 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BQ.1.33 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.29 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.5.1.28 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CL.1.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HH.8.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.19 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FM.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FK.1.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.2.7.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.8.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| EU.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.14 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.21 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| EJ.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| KD.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XCS | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GA.7.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.15.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.2.40 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CR.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| KB.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GK.11 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CJ.1.3.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XAN | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BN.1.4.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| DV.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.5.2.11 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.2.53 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -6316.86 | Japan |
| BA.4.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| EU.1.1.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| CM.2.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.43 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HU.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JB.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.5.55 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBP | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |