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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.5.2.56 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| KP.2.13 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -22762.7 | Japan |
| XDP.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -22762.7 | Japan |
| HN.8 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| KL.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| HK.24.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | 1233.44 | Japan |
| GK.2.4 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | 1233.44 | Japan |
| FL.1.5.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| HE.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| JM.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| HV.1.5 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.5.11 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BQ.1.1.27 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BF.7.22 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| GP.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| XBB.3.4 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| XBV | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BQ.1.1.62 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| GA.7 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| FL.23 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| XBB.2.3.13 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.110.3 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| AY.23 (Delta) | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| AY.9 (Delta) | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| AY.74 (Delta) | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.621.1 (Mu) | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.2.5 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BF.7.5 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BF.32 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BQ.1.1.67 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| CK.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BE.1.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.5.1.38 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.5.2.34 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BF.7.12 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| CJ.1.2 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BQ.1.1.21 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.4.6.3 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| CM.7.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BA.5.3.3 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BQ.1.22 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| BF.7.19 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| FA.1 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.1.464 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.234 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.1.231 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.243 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.517 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| B.1.1.291 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -2091.76 | Japan |
| B.1.258 | S | WuhanCoV-spk1 | TTGGCAAAATTCAAGACTCACTTT | 24 | 33.33 | 24354 | 24377 | F | -23366.4 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |