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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.38.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XAV | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BE.1.4.4 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBC.1.3 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BM.4.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BF.20 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BA.2.77 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| CP.1.3 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| FC.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BY.1.2 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| AZ.2 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.97 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.36.18 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.177.32 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.59 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.177.67 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.277 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -2834.99 | Japan |
| AY.28 (Delta) | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.272 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.371 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -4248.57 | Japan |
| AA.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.86 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.154 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.4.8 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| A.2.4 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.41 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.137 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.446 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| AY.76 (Delta) | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.398 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.163 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| P.3 (Theta) | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.254 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| AY.39.1.1 (Delta) | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.418 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.9.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.40 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.122 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XAJ | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.431 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.168 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| Q.6 (Alpha) | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -1590.7 | Japan |
| B.1.1.41 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.527 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| C.18 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.500 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.147 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.1.487 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| B.1.177.68 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -4248.57 | Japan |
| B.1.1.121 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |