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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| KS.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| JN.1.16.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LW.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LP.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.2.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.3.3 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| ML.2 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.2.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.1.6 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.3 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.1.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.4 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| MM.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| JN.1.48.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.4.2.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| MC.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LP.1.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| JN.1.50.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| MH.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.3.2 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.3.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.4 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.2 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.2.6 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| MA.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| KP.3.1.4 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| LB.1.8 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBB.1.5.81 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| CH.1.1.20 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBL.3.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBB.2.4 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBB.1.28.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| GA.6.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBB.1.5.61 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| FR.1.2 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| FL.2.2.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -21537 | China |
| FL.25 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| FL.33 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| FL.29 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| EG.13 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| GP.1 | E | Huang-E | ACTTCTTTTTCTTGCTTTCGTGGT | 24 | 37.5 | 26295 | 26318 | F | -25022.8 | China |
| XBB.1.28.1 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| GA.6.1 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| XBB.1.5.61 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| FR.1.2 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| FL.2.2.1 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| FL.25 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| FL.33 | N | Young-N | AGCACCATAGGGAAGTCC | 18 | 55.56 | 28648 | 28631 | R | -19874.5 | Singapore |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |