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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| GK.6 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| EG.5.2.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HW.1.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HN.7 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GE.1.6 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| EG.5.1.9 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XCH | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -301.668 | South Korea |
| JF.3 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GK.1.5 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| DV.7.1.7 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| DV.7.1.3 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JP.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XCT.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.15 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GS.7 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FY.5.4 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FE.1.1.5 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.20 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GK.12 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.2.3.8 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FL.24 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.2.3.20 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| EG.5.2.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.27 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.30 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| EG.5.1.17 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| DV.6.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FK.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HF.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBC.1.6 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FL.1.4 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FY.4.1.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HH.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GY.7 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FY.5.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HH.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.1.5.85 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FU.5 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| FY.3.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.86 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.1.41.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| GS.4.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JF.4 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| EG.5.1.13 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JG.3.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.1.16.28 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JN.1.13 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XBB.1.16.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |