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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBJ.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.356 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.462 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| A.29 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.147 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.6 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.5 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.4 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.420 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| B.1.428 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.355 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.34.1.1 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.71 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.33.2 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.177.52 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| B.1.465 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.160.33 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.4.2.5 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XF | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.127.1 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.88 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| AP.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AK.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.98.1.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BA.1.13.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| HK.34.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.124.1.1 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.203 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| FY.8.1.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GY.9 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.160.25 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.1.5.74 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.7 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BN.2.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.2.6.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.238 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.1.7 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.1.11 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BQ.1.1.68 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BF.7.26 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| N.4 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AM.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.15 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.113 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GA.10 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BF.5.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.423 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.550 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.261 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.3.5 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |