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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| AY.11 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| GY.9 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.7.1 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.254 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.36.1 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.36.12 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.177.21 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.122.2 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.79 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AQ.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.1.1.7 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.209 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.428 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.355 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.34.1.1 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.71 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.33.2 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.177.52 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.465 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.160.33 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| AY.4.2.5 (Delta) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XF | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| S.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.221.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.258.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.215 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.36.20 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.177.82 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.462 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.218 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.177.74 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.2.25 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.237 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XBN | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XBJ.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.356 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.462 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| A.29 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.147 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| P.1.6 (Gamma) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| P.1.5 (Gamma) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| P.1.4 (Gamma) | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.420 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XBB.2.12 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BF.39.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| EK.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.233 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XBB.1.24 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.320 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| C.33 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |