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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| LZ.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KP.3.1.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KW.1.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KU.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| JN.1.4.6 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KP.2.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| LP.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KP.3.1.3 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| GQ.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| JA.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| EG.5.1.10 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| FL.2.7.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| EG.9.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| XBB.2.3.22 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| JV.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.2.3.4 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| CH.1.1.7 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| XBB.1.27 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BQ.1.10.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BQ.1.1.36 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| EF.1.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BQ.1.32 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| EK.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| XBB.2.6.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| DV.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| FL.23.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| XBB.1.5.64 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.2.68 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| AY.65 (Delta) | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.466 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| L.4 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.1.339 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.229 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.415 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.1.370 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.421 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.428.3 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.612 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| B.1.305 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KP.3.2.4 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| JN.1.50 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KP.2.14 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| unclassifiable | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| KS.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| FL.1.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| FY.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| HH.2.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| XCE | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| HN.4.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| GU.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |