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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| AY.116.1 (Delta) | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| AY.52 (Delta) | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| AY.13 (Delta) | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| AY.35 (Delta) | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| AY.122 (Delta) | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XDK.1.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.5.20 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LB.1.7.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.18.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.16.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LR.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KR.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LQ.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XDK.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.4.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JF.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GK.1.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JD.1.1.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| HK.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5.1.14 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.7.9 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KW.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| MF.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LF.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KR.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.7.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KP.2.3.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KR.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.49 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| MF.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.29.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| KP.2.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.11 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.24.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.18.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LU.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.40 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.2.3.19 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JD.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XDA | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5.1.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JG.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5.1.6 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GK.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.1.5.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JF.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| MG.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| JN.1.59 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| LG.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |