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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
XBB.1.5.27	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.2.3.17	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.2.3.17	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
FD.5	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
FD.5	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.44	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.1.44	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.5.26	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.1.5.26	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
GN.3	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
GN.3	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
FW.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
FW.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
FY.1.2.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
FY.1.2.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.636	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.636	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.126	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.126	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BQ.1.1.47	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BQ.1.1.47	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XDQ.3	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
XDQ.3	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
JD.1.1.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
JD.1.1.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBL	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBL	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
EK.3	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
EK.3	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.466.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.466.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.177	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.177	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
C.36	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
C.36	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.222	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.222	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.470	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
B.1.470	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.31 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.31 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.51 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.51 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.122.5 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.122.5 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.2.40.1	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.2.40.1	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.28	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.1.28	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
GK.3	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used