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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
XBB.1.30	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
DT.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
DT.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
DT.1	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
DT.1	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XCA	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XCA	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.5.50	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.1.5.50	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BQ.1.1.54	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BQ.1.1.54	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
GN.1.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
GN.1.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.77 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.77 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.53 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.53 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.36.10	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.36.10	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.599	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.599	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.128	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.128	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.451	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.451	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.8	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.8	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.313	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.313	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.402	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.402	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.609	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.609	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.344	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.344	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.556	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.556	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.245	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.245	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.403	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.403	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBC.1.1	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBC.1.1	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
FY.9	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
FY.9	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.31.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
XBB.1.31.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
GK.1.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
GK.1.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XBB.1.5.27	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used