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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.213	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.56 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.56 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.39.1.4	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.39.1.4	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.91.1 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.91.1 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.4.14 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.4.14 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
B.1.1.359	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
B.1.1.359	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.1.3	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-11626.3	Germany
BA.1.3	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-3196.01	Germany
XBC.2	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
XBC.2	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
Q.2 (Alpha)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
Q.2 (Alpha)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BL.1.1	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-19379.1	Germany
BL.1.1	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
M.3	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
M.3	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
C.2	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
C.2	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.45 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.45 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.70 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.70 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.26.1 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.26.1 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.5 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.5 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.108 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.108 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.32 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.32 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.46.3 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.46.3 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.4.6 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.4.6 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.75.2 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
AY.75.2 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
C.37 (Lambda)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
C.37 (Lambda)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
P.1.14 (Gamma)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
P.1.14 (Gamma)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
P.1.12 (Gamma)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
P.1.12 (Gamma)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
B.1.525 (Eta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
B.1.525 (Eta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.6 (Delta)	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-24645.6	Germany
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used