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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
BQ.1.1.26	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BQ.1.1.26	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BQ.1.1.2	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BQ.1.1.2	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
XBB.1.1	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
XBB.1.1	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
FK.1.1.2	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
FK.1.1.2	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
FT.3	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
FT.3	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
XBF.5	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
XBF.5	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
GJ.1.2.5	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
GJ.1.2.5	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.1.25	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.1.25	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
XAG	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
XAG	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
DJ.1.1	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
DJ.1.1	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BQ.1.16	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BQ.1.16	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.1.21	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.1.21	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.1.12	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.1.12	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BF.11.2	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BF.11.2	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
EE.1	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
EE.1	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
B.1.177.85	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
B.1.177.85	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.66 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.66 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.72 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.72 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.133 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.133 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.5.1 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.5.1 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.43.7 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.43.7 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
FY.4	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
FY.4	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
B.1.497	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
B.1.497	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
EN.1.1	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
EN.1.1	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.30 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.30 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used