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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BY.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BY.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BC.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BC.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| P.1.17.1 (Gamma) | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| P.1.17.1 (Gamma) | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| BA.2.3.16 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.3.16 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| B.1.177.41 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| B.1.177.41 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.1.375 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| B.1.1.375 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| CM.8.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CM.8.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBS | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| XBS | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BN.1.2.8 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.2.8 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XAH | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| XAH | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.5.2.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -18184 | Thailand |
| BA.5.2.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| XBJ.3 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -18184 | Thailand |
| XBJ.3 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| B.1.177.62 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| B.1.177.62 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| BA.2.82 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.82 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBB.1.20 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| XBB.1.20 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.3.22 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.3.22 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.38.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.38.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.79.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.79.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BL.1.4 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BL.1.4 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.75.9 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.75.9 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBH | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| XBH | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BL.1.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BL.1.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BQ.1.1.78 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1.78 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| B.1.9.4 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| B.1.9.4 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| BQ.1.1.64 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1.64 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |