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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBB.1.5.79 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.79 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.3 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.3 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| GG.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| GG.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| FL.3.2 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| FL.3.2 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| CK.1.5 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| CK.1.5 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.34 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.34 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| GD.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| GD.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.52 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.52 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| CH.1.1.27 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| CH.1.1.27 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.65 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.65 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.75 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.75 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.21 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -17554.7 | Japan |
| XBB.1.5.21 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.18.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.18.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.24 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BQ.1.24 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| GL.3 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| GL.3 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.1.24 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BQ.1.1.24 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.78 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.78 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| EG.1.3 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| EG.1.3 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.1.45 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BQ.1.1.45 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.37.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.37.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.77 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.77 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| FD.4.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| FD.4.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.5.51 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.5.51 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBB.1.16.8 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| XBB.1.16.8 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.2.1 | N | NIID-JP-N | AAATTTTGGGGACCAGGAAC | 20 | 45 | 29125 | 29144 | F | -20489.8 | Japan |
| BQ.1.2.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |