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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
A.2.5	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.102 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.102 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
B.1.177.31	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.177.31	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
B.1.160.26	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.160.26	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.39.1.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.39.1.2 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.4.15 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.4.15 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.2.75.10	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-15016.3	South Korea
BA.2.75.10	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.122.4 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.122.4 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.101 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.101 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.1.5	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.1.5	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.1.14	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.1.14	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.2.57	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.2.57	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.2.49	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.2.49	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.124 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.124 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.46.6 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.46.6 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.43.3 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.43.3 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.119.1 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.119.1 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.25.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.25.2 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.122.6 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.122.6 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
B.1.621.2	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.621.2	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.39.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.39.2 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.125 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.125 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.103.1 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.103.1 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
AY.92 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.92 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.2.9.2	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
BA.2.9.2	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
BA.2.11	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-18253.7	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used