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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.179 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| C.3 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| C.3 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BQ.1.8.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BQ.1.8.1 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.12 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.12 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| AY.75.3 (Delta) | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| AY.75.3 (Delta) | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BF.35 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BF.35 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| CY.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| CY.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| FR.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FR.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.483 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.483 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.1.181 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.181 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.113 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.113 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.594 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.594 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.328 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.328 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.5 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.5 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.1.283 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.283 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BE.12 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BE.12 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.96 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.96 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| L.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| L.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.310 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.310 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BN.1.2.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BN.1.2.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| CQ.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| CQ.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| CH.1.1.24 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| CH.1.1.24 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BA.5.2.43 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BA.5.2.43 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BA.5.2.19 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| BA.5.2.19 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| BF.38.3 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -19060.5 | South Korea |
| BF.38.3 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.351.3 (Beta) | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |