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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.85 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.65 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.65 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.1.3 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.5.1.3 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BG.5 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BG.5 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.10.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.10.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.3.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.3.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.3.13 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.3.13 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.3.9 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.3.9 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.10.2 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.10.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.56 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.56 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.61 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -2052.38 | South Korea |
| BA.2.61 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.1.23 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -2052.38 | South Korea |
| BA.5.1.23 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BF.8 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BF.8 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.74 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.74 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.12 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.12 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BM.3 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -19758.2 | South Korea |
| BM.3 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.2.28 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.5.2.28 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.2.62 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.5.2.62 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.2.24 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -2052.38 | South Korea |
| BA.5.2.24 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.8 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -2052.38 | South Korea |
| BA.5.8 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.2.13.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.2.13.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BF.28 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BF.28 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BA.5.1.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BA.5.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BE.1.1.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| BE.1.1.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XAZ | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| XAZ | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| BE.4 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |