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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
BA.2.20	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.2.73	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.2.73	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
XDZ	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-15341.9	South Korea
XDZ	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-301.668	South Korea
XEB	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
XEB	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.263	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.263	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.9 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.9 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
AY.74 (Delta)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
AY.74 (Delta)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.621.1 (Mu)	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.621.1 (Mu)	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.2.5	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.2.5	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BF.7.5	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BF.7.5	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BF.32	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-19758.2	South Korea
BF.32	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.180	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.180	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.464	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.464	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.234	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.234	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.231	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.231	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.243	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.243	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.517	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.517	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.291	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.291	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.258	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.258	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.349	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.349	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.314	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.314	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.1.132	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
B.1.1.132	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.2.38	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.2.38	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
BA.2.42	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
BA.2.42	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
K.2	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
K.2	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
JN.1.56	ORF1ab	Won-ORF1ab	CATGTGTGGCGGTTCACTAT	20	50	15441	15460	F	-22753.7	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used