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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.354 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.162 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.162 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.426 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.426 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.402 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.402 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.116 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.116 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.496 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.496 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.438.4 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.438.4 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.49 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.49 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.507 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.507 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.294 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.294 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.453 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.453 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.44 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.44 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.350 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.350 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.304 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.304 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.177 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.177 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.301 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.301 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.308 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.308 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.210 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.210 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.325 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.325 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.590 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.590 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FP.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| FP.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| BA.2.2.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.2.2.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| BA.2.2 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.2.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.443 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.443 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.595.4 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.595.4 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.495 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |