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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XCT.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| XCT.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HK.15 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HK.15 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GS.7 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| GS.7 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.16.27 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| XBB.1.16.27 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GY.1.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| GY.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HH.8 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HH.8 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HK.25 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HK.25 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EG.5.1.11 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| EG.5.1.11 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HK.9 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HK.9 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FL.13.4.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| FL.13.4.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EG.5.1.15 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| EG.5.1.15 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JY.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| JY.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.16.24 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| XBB.1.16.24 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HK.17 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HK.17 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| DV.7.1.7 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| DV.7.1.7 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FL.15.4 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| FL.15.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JR.1.1.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| JR.1.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JD.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| JD.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JG.3.3 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| JG.3.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KE.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| KE.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HV.1.2 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HV.1.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.2.3.15 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| XBB.2.3.15 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GK.2.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| GK.2.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HK.1.2 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HK.1.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HN.4.2 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| HN.4.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |