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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.87 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.2.87 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.4.8 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.4.8 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -10838.6 | Germany |
| B.1.617.1 (Kappa) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.617.1 (Kappa) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.4.3 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.4.3 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -10838.6 | Germany |
| AY.46.5 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.46.5 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.4.1 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.4.1 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -10838.6 | Germany |
| P.1.16 (Gamma) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| P.1.16 (Gamma) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.220 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.220 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.58 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.58 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.258.6 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.258.6 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.438 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.438 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.257 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.257 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.5.4 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.5.4 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.275 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.275 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.258.2 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.258.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.177.58 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.58 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.177.81 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.81 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.419 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.419 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.151 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.151 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.469 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.469 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| BA.2.62 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -3521.26 | Germany |
| BA.2.62 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -5057.65 | Germany |
| AY.80 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.80 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.43.8 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.43.8 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.128 (Delta) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -17266.7 | Germany |
| AY.128 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| A.21 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| A.21 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |