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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.25 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.1.25 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.189 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.189 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.563 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.563 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.36.34 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.36.34 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.5.3 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.5.3 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BB.2 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| BB.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.86 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.86 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.4.7 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.4.7 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.4.12 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.4.12 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.210 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.210 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| A.28 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| A.28 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.567 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.567 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| C.23 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| C.23 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| C.2.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| C.2.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.177.54 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.177.54 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.164 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.164 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KY.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| KY.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JN.2.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| JN.2.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.392 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.1.392 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.377 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.377 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.177.25 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.177.25 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.23.2 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.23.2 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| AY.104 (Delta) | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| AY.104 (Delta) | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.198 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| B.1.198 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EG.1.1 | N | Chan-N | GCGTTCTTCGGAATGTCG | 18 | 55.56 | 29210 | 29227 | F | -21207.6 | China |
| EG.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |