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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.8 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.2.8 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.7.11 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BF.7.11 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CE.1 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| CE.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.2.6 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.2.6 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.3.2 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.5.3.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.2.7 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.5.2.7 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BE.2 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BE.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BQ.1.1.9 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BQ.1.1.9 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.11.3 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BF.11.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBF.3 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| XBF.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BZ.2 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BZ.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BQ.1.10.1 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BQ.1.10.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.7.24 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BF.7.24 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.10 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.5.10 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.2.44 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.2.44 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BE.1.4 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BE.1.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| EA.1 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| EA.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.2.22 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BA.2.22 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| DH.1 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| DH.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XG | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| XG | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.6 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| BF.6 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| GN.2 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| GN.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FY.1.2.1 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| FY.1.2.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.413 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| B.1.413 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FY.5.5 | ORF1ab | Yip-ORF1ab | ATGCATTTGCATCAGAGGCT | 20 | 45 | 1866 | 1885 | F | -21402.5 | China |
| FY.5.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |