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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.621.1 (Mu) | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BA.2.5 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BA.2.5 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BF.7.5 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BF.7.5 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BF.32 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BF.32 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.1.180 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.1.180 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.1.464 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.1.464 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.234 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.234 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.1.231 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.1.231 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.243 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.243 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| CH.1.1.11 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| CH.1.1.11 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| FJ.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| FJ.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BN.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BN.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| DV.3.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| DV.3.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| XBB.1.5.14 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| XBB.1.5.14 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| XBB.1.5.80 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| XBB.1.5.80 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BQ.1.1.23 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BQ.1.1.23 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BA.5.2.18 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BA.5.2.18 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BQ.1.11 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BQ.1.11 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BQ.1.1.3 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BQ.1.1.3 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| EG.4.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| EG.4.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BQ.1.1.17 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BQ.1.1.17 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| DF.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| DF.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| CK.1.3 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| CK.1.3 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| BA.5.2.27 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| BA.5.2.27 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| XBB.1.4 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| XBB.1.4 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| XBB.1.5.16 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |