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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.458 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.1.17 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.17 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.258.22 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.258.22 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| AG.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| AG.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.258.16 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.258.16 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| W.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| W.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.264.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.264.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.190 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.190 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.552 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.552 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.1.396 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.396 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.1.129 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.129 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| AK.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| AK.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| C.20 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| C.20 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.221.4 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.221.4 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.1.224 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.224 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| BA.4.6.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.4.6.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XBJ | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBJ | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| EJ.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| EJ.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XBB.1.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.3 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| DQ.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| DQ.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| BF.31.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BF.31.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.1.152 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.152 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.146 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.146 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| D.5 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| D.5 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.81 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.81 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| B.1.258.24 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |