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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.332 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.1.332 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JB.2.1 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| JB.2.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.2.86.7 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BA.2.86.7 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -26378.9 | Japan |
| B.1.12 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.12 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JW.1.1 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| JW.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.2.3.18 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| XBB.2.3.18 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBC.1.6.5 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| XBC.1.6.5 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| AH.2 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| AH.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.1.280 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.1.280 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.597 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.597 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.177.45 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.177.45 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.177.72 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.177.72 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| AY.43.7 (Delta) | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| AY.43.7 (Delta) | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| AY.33.1 (Delta) | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| AY.33.1 (Delta) | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XD | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| XD | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.493 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.493 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.351.2 (Beta) | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.351.2 (Beta) | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.640.2 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.640.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.1.352 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.1.352 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.1.1.251 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.1.251 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HC.1 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| HC.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XCJ | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| XCJ | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FT.2 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| FT.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FZ.2 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| FZ.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -20723.1 | Japan |
| B.1.177.43 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| B.1.177.43 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |