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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
M.3	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
M.3	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
C.2	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
C.2	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.45 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.45 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.70 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.70 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.26.1 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.26.1 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.5 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.5 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.108 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.108 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.32 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.32 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.46.3 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.46.3 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.4.6 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.4.6 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.75.2 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.75.2 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
C.37 (Lambda)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
C.37 (Lambda)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
P.1.14 (Gamma)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
P.1.14 (Gamma)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
P.1.12 (Gamma)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
P.1.12 (Gamma)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
B.1.525 (Eta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
B.1.525 (Eta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.6 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.6 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.5.5 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.5.5 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
P.1.7 (Gamma)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
P.1.7 (Gamma)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
AY.24 (Delta)	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
AY.24 (Delta)	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BN.1.7	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BN.1.7	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BF.5.5	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BF.5.5	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
XBB.1.41	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
XBB.1.41	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.2.75.2	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.2.75.2	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
BA.5.1.20	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
BA.5.1.20	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
CR.1.3	ORF1ab	HKU-ORF1b	TGGGGYTTTACRGGTAACCT	20	45	18778	18797	F	-7043.36	Hong Kong
CR.1.3	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used