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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| JQ.2.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KT.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| KT.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| LA.2 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| LA.2 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| LH.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| LH.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.10 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.10 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| GW.5.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| GW.5.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.53.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.53.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| XDQ.2 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| XDQ.2 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HV.1.11 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HV.1.11 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KA.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| KA.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HF.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HF.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HK.22 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HK.22 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| FW.1.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| FW.1.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.13 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.13 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KH.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| KH.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| LM.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| LM.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.17 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.17 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.15 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.15 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.1.2 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.1.2 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.63.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.63.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.27 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.27 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.12 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.12 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.51 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.51 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.1.9 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -893.215 | Hong Kong |
| JN.1.1.9 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.19 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.19 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KU.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |