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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BF.7 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| BF.7 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BF.7 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -22073.5 | China |
| BF.7 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.7 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| BF.7 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BF.7 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| BF.7 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| BA.2.23 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| BA.2.23 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BA.2.23 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -22073.5 | China |
| BA.2.23 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.2.23 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| BA.2.23 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BA.2.23 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| BA.2.23 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| BA.5.2.44 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -1313 | China |
| BA.5.2.44 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BA.5.2.44 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -3043.11 | China |
| BA.5.2.44 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.5.2.44 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| BA.5.2.44 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BA.5.2.44 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| BA.5.2.44 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| BN.1.3.9 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| BN.1.3.9 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BN.1.3.9 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -22073.5 | China |
| BN.1.3.9 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| BN.1.3.9 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| BN.1.3.9 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BN.1.3.9 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| BN.1.3.9 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| BQ.1.1.8 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -2551.95 | China |
| BQ.1.1.8 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -2518.95 | Hong Kong |
| BQ.1.1.8 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -3043.11 | China |
| BQ.1.1.8 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.1.8 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| BQ.1.1.8 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| BQ.1.1.8 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| BQ.1.1.8 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| DV.6 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| DV.6 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| DV.6 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -22073.5 | China |
| DV.6 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| DV.6 | N | US-CDC-N-3 | TGTAGCACGATTGCAGCATTG | 21 | 47.62 | 28752 | 28732 | R | -23779.6 | US |
| DV.6 | ORF1ab | NIID WH-1 F501R913 | TTCGGATGCTCGAACTGCACC | 21 | 57.14 | 484 | 504 | F | -24908.9 | Japan |
| DV.6 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| DV.6 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| BA.2 (Omicron) | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| BA.2 (Omicron) | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -2518.95 | Hong Kong |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |