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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| AY.2 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| B.1.621 (Mu) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.621 (Mu) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.9.2 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.9.2 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.34 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.34 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.47 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.47 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| P.1.17 (Gamma) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| P.1.17 (Gamma) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.126 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.126 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| XB | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XB | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.48 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.48 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.116.1 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.116.1 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.52 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.52 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.13 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.13 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.35 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.35 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.122 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.122 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| B.1.1.7 (Alpha) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| B.1.1.7 (Alpha) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| P.1 (Gamma) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| P.1 (Gamma) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.120.1 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.120.1 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| AY.119.2 (Delta) | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| AY.119.2 (Delta) | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -24645.6 | Germany |
| GS.8 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GS.8 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| GE.1.4 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GE.1.4 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| XBB.1.5.93 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XBB.1.5.93 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| FL.2.3.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FL.2.3.1 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| FL.4.8 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FL.4.8 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| EG.5.1.18 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| EG.5.1.18 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| HK.4 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.4 | E | Charité-E | ACAGGTACGTTAATAGTTAATAGCGT | 26 | 34.62 | 26269 | 26294 | F | -19379.1 | Germany |
| GM.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |