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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
BA.2.16	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.16	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.16	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.16	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.16	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-14622	Hong Kong
BA.2.16	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.9.4	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.2.9.4	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.2.9.4	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.9.4	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.9.4	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.9.4	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.9.4	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-18800.2	Hong Kong
BA.2.9.4	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.55	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.2.55	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.2.55	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.55	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.55	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.55	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.55	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-18800.2	Hong Kong
BA.2.55	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.39	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.2.39	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.2.39	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.39	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.39	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.39	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.39	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-18800.2	Hong Kong
BA.2.39	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.64	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-20262.1	Germany
BA.2.64	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.2.64	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.64	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.64	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.64	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.64	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-18800.2	Hong Kong
BA.2.64	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.8 (Delta)	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-17266.7	Germany
AY.8 (Delta)	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
AY.8 (Delta)	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-21977.3	China
AY.8 (Delta)	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
B.1.469	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.62	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-3521.26	Germany
BA.2.62	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
BA.2.62	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-17926.1	China
BA.2.62	N	CN-CDC-N	CAGACATTTTGCTCTCAAGCTG	22	45.45	28979	28958	R	-23140.2	China
BA.2.62	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	-22824.1	US
BA.2.62	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.62	N	HKU-N	TAATCAGACAAGGAACTGATTA	22	31.82	29145	29166	F	-18800.2	Hong Kong
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used