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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage
(WHO label)
  
Target Gene
  
Primer Name
  
Primer Sequence
  
Primer Length
  
Primer GC Content (%)
  
Start Position
  
End Position
  
Primer Direction
  
ΔG (cal)
  
Primer first used
  
JC.4NWon-NGTTGCGACTACGTGATGAGG20552884928830R-23088.1South Korea
JC.4NNIID-JP-NAAATTTTGGGGACCAGGAAC20452912529144F-20489.8Japan
JC.4NNIID-JP-NTGGCAGCTGTGTAGGTCAAC20552928229263R-20522.3Japan
JC.4ORF1abNIID WH-1 Seq F519R840ACCTCATGGTCATGTTATGG2045502521F-20812.9Japan
B.1.564ORF1abYip-ORF1abATGCATTTGCATCAGAGGCT204518661885F-21402.5China
B.1.564ORF1abYip-ORF1abTTGTTATAGCGGCCTTCTGT204519701951R-21075.5China
B.1.564ORF1abPasteur-ORF1ab-2CTGGTCAAGGTTAATATAGG20401418614167R-18348.8France
B.1.564ORF1abWon-ORF1abCATGTGTGGCGGTTCACTAT20501544115460F-22753.7South Korea
B.1.564ORF1abWon-ORF1abTGCATTAACATTGGCCGTGA20451555815539R-21175.8South Korea
B.1.564ORF1abChan-ORF1abCGCATACAGTCTTRCAGGCT20501622016239F-11502.5China
B.1.564ORF1abHKU-ORF1bTGGGGYTTTACRGGTAACCT20451877818797F-7043.36Hong Kong
B.1.564SYoung-STATACATGTCTCTGGGACCA20452176321782F-19121.1Singapore
B.1.564SWon-SCTACATGCACCAGCAACTGT20502311423133F-22142.8South Korea
B.1.564SWon-SCACCTGTGCCTGTTAAACCA20502321323194R-21965.9South Korea
B.1.564EWon-ECACACAATCGATGCGCAGTA20502636526346R-19261.4South Korea
B.1.564EHuang-EGCAGCAGTACGCACACAATC20552637626357R-23540.5China
B.1.564ENiu-ETTCTTGCTTTCGTGGTATTC20402630326322F-19913.4China
B.1.564ENiu-ECACGTTAACAATATTGCAGC20402639126372R-20723.8China
B.1.564NNIH-TH_NCGTTTGGTGGACCCTCAGAT20552832028339F-22019.3Thailand
B.1.564NUS-CDC-N-1GACCCCAAAATCAGCGAAAT20452828728306F-20656.7US
B.1.564NUS-CDC-N-2TTACAAACATTGGCCGCAAA20402916429183F-21115.5US
B.1.564NCorman-NGAGGAACGAGAAGAGGCTTG20552883328814R-19636.2Germany
B.1.564NWon-NCAATGCTGCAATCGTGCTAC20502873228751F-22388.1South Korea
B.1.564NWon-NGTTGCGACTACGTGATGAGG20552884928830R-23088.1South Korea
B.1.564NNIID-JP-NAAATTTTGGGGACCAGGAAC20452912529144F-20489.8Japan
B.1.564NNIID-JP-NTGGCAGCTGTGTAGGTCAAC20552928229263R-20522.3Japan
B.1.564ORF1abNIID WH-1 Seq F519R840ACCTCATGGTCATGTTATGG2045502521F-20812.9Japan
B.1.1.461ORF1abYip-ORF1abATGCATTTGCATCAGAGGCT204518661885F-21402.5China
B.1.1.461ORF1abYip-ORF1abTTGTTATAGCGGCCTTCTGT204519701951R-21075.5China
B.1.1.461ORF1abPasteur-ORF1ab-2CTGGTCAAGGTTAATATAGG20401418614167R-18348.8France
B.1.1.461ORF1abWon-ORF1abCATGTGTGGCGGTTCACTAT20501544115460F-22753.7South Korea
B.1.1.461ORF1abWon-ORF1abTGCATTAACATTGGCCGTGA20451555815539R-21175.8South Korea
B.1.1.461ORF1abChan-ORF1abCGCATACAGTCTTRCAGGCT20501622016239F-11502.5China
B.1.1.461ORF1abHKU-ORF1bTGGGGYTTTACRGGTAACCT20451877818797F-7043.36Hong Kong
B.1.1.461SYoung-STATACATGTCTCTGGGACCA20452176321782F-19121.1Singapore
B.1.1.461SWon-SCTACATGCACCAGCAACTGT20502311423133F-22142.8South Korea
B.1.1.461SWon-SCACCTGTGCCTGTTAAACCA20502321323194R-21965.9South Korea
B.1.1.461EWon-ECACACAATCGATGCGCAGTA20502636526346R-19261.4South Korea
B.1.1.461EHuang-EGCAGCAGTACGCACACAATC20552637626357R-23540.5China
B.1.1.461ENiu-ETTCTTGCTTTCGTGGTATTC20402630326322F-19913.4China
B.1.1.461ENiu-ECACGTTAACAATATTGCAGC20402639126372R-20723.8China
B.1.1.461NNIH-TH_NCGTTTGGTGGACCCTCAGAT20552832028339F-22019.3Thailand
B.1.1.461NUS-CDC-N-1GACCCCAAAATCAGCGAAAT20452828728306F-20656.7US
B.1.1.461NUS-CDC-N-2TTACAAACATTGGCCGCAAA20402916429183F-21115.5US
B.1.1.461NCorman-NGAGGAACGAGAAGAGGCTTG20552883328814R-22527.5Germany
B.1.1.461NWon-NCAATGCTGCAATCGTGCTAC20502873228751F-22388.1South Korea
B.1.1.461NWon-NGTTGCGACTACGTGATGAGG20552884928830R-23088.1South Korea
B.1.1.461NNIID-JP-NAAATTTTGGGGACCAGGAAC20452912529144F-20489.8Japan
B.1.1.461NNIID-JP-NTGGCAGCTGTGTAGGTCAAC20552928229263R-20522.3Japan
B.1.1.461ORF1abNIID WH-1 Seq F519R840ACCTCATGGTCATGTTATGG2045502521F-20812.9Japan
Lineage
(WHO label)
  
Target Gene
  
Primer Name
  
Primer Sequence
  
Primer Length
  
Primer GC Content (%)
  
Start Position
  
End Position
  
Primer Direction
  
ΔG (cal)
  
Primer first used