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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| EG.13 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| GP.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| HL.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XCD | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.1.44.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| EG.2.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.37 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.1.45.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FP.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.1.5.102 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FK.1.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| GN.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.31 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.13.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| EG.7 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BN.1.3.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XCF.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XCC | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FK.1.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.21.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FP.2.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.1.5.92 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.1.5.42 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| FL.35.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| GE.1.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.2.54 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| DR.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.7.27 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| B.1.1.26 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CM.8 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.1.27 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.16 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.5.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.2.59 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BF.15 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BQ.1.15 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CA.5 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CP.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XBB.3.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| ED.3 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CM.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BQ.1.4 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| CA.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BN.1.9 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BQ.1.28 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| DG.1 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| BA.5.2.56 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| KP.2.13 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| XDP.2 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| HN.8 | ORF1ab | Yip-ORF1ab | TTGTTATAGCGGCCTTCTGT | 20 | 45 | 1970 | 1951 | R | -21075.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |