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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| GE.1.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.5.93 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.2.3.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.4.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5.1.18 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| HK.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GM.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.13.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GV.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.5.25 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BN.1.2.6 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.16.26 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GJ.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| CM.12 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| DY.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BF.7.14.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.3.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| DY.1.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| HH.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EM.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FD.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GF.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| EG.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.5.24 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FU.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.4.6 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GY.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BF.7.14.6 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BF.7.14.1 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BN.1.3.10 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| DY.2 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BA.5.2.48 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BA.5.2.49 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BF.7.14.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.2.3.11 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.10 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.4 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.4.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| GY.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.5.57 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.35 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FL.5 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| FU.3 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| XBB.1.16 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BN.1.3.6 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| BN.1.3.8 | S | NIID WH-1 Seq F24383R24865 | CAAAGACACCTTCACGAGG | 19 | 52.63 | 24848 | 24830 | R | -20677.6 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |