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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BE.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.1.1.15 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BF.7.16 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BA.2.3.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CQ.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| EC.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.5.2.52 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BA.2.3.4 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CH.1.1.7 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBB.1.27 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BQ.1.10.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BQ.1.1.36 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| EF.1.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BQ.1.32 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| EK.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBB.2.6.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| DV.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| FL.23.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| XBB.1.5.64 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.68 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| AY.65 (Delta) | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.466 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| L.4 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.1.339 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.229 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.415 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.1.370 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.421 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.428.3 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.612 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.305 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| FY.1.2.3 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| GK.1.11.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3277.98 | Thailand |
| FL.32.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| GA.8.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| HK.34 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| HC.3 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| B.1.1.512 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.1.349 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.265 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.401 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| B.1.1.362 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -22126.5 | Thailand |
| BA.1.6 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| GK.2.3 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| FW.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| HN.3.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.1.1.17 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BS.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CM.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.75.7 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |