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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBJ.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.356 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.462 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| A.29 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.147 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.6 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.5 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| P.1.4 (Gamma) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.420 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| XBB.2.12 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BF.39.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| EK.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.233 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| XBB.1.24 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.320 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| C.33 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.635 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| C.21 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| C.14 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.19 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| N.4 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AM.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.15 (Delta) | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.113 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| GA.10 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| BF.5.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.423 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.494 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.167 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| B.1.267 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| B.1.165 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| B.1.554 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.243.2 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| A.2.5.3 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.450 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| AY.130 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.444 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -3270.88 | China |
| AW.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.268 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.344 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.103 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.485 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.119 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.276 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.111 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.36.27 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.36.19 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.1.336 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.400.1 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| B.1.537 | ORF1ab | CN-CDC-ORF1ab | ACGATTGTGCATCAGCTGA | 19 | 47.37 | 13460 | 13442 | R | -20306.2 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |