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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| FL.17.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| EG.12 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| CM.7.1.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FL.10.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FD.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| KG.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.5.13 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| HA.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.351 (Beta) | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.5.36 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| HZ.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.5.30 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FL.19.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.5.100 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.16.19 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.43.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| EU.1.1.3 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XBB.1.5.18 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.443 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.595.4 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.495 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.294 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.453 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.44 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.350 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.304 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.315 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.516 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.354 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.162 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.426 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.402 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.424 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.61 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.264 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.581 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.330 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.205 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.177 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.301 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.308 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.210 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.1.325 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.590 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.116 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.496 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| B.1.438.4 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| AY.49 (Delta) | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FZ.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| FY.4 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |