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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBL | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| EK.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| B.1.466.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| B.1.177 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| C.36 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| B.1.222 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| B.1.470 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| AY.31 (Delta) | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| AY.51 (Delta) | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| AY.122.5 (Delta) | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.2.40.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XBB.1.28 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| GK.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| FL.13.5 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| FL.10.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| GS.6 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| HG.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| B.1.323 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| FU.1.1.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| FY.8 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| FN.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BQ.1.1.74 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XBB.1.5.53 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| JB.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| A.4 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XDK.4 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.2.58 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.4.1.6 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.2.3.6 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| GA.10.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.2.3.14 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| CP.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BQ.1.1.39 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -21745 | Germany |
| BF.40 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BN.1.3.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BM.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BQ.1.1.57 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| EF.1.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BQ.1.29 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.5.1.32 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BW.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XBB.9 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| CK.1.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| CM.8.1.3 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| CA.3.1 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BA.5.2.61 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XBB.2.5 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| ED.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| BU.2 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| XBB.1.6 | N | Corman-N | GAGGAACGAGAAGAGGCTTG | 20 | 55 | 28833 | 28814 | R | -22527.5 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |